ClaricepTMFlash positive phase silicone has SiO4Tetrahedral structure, active hydrophilic surface. Water molecules form acidic silica gel through hydrogen bonding with exposed surfaces. The porous properties and silanol functional groups make silica gel an economical chromatographic separation material that can separate weakly polar compounds, which requires the use of a less polar mobile phase.
ClaricepTMFlash silicone column
Using ultra pure silica filling (washed with acid and deionized water, with a narrow particle size range and precise control of moisture content)
Specific surface area: 480 m2/g. Aperture: 60 Å; pH： 6.3-7.2； Moisture content: 3% -5%; Average particle size: 40-60 μ m;
(1) ClaricepTMFlash silicone column
Ultra pure silicone (specially acid washed, with narrow particle size distribution and consistent moisture content)
Aniline testing
ClaricepTMFlash CS 4.09 min
Sample: Acetophenone, 4-aminobenzoic acid
Mobile phase: n-hexane/ethyl acetate (gradient elution)
Detection wavelength: 254 nm
(2) ClaricepTMFlash activated silicone column
Bonaijer has developed a special deactivated silicone filler. Compared with ordinary silica gel columns, it significantly improves performance, controls the balanced surface activity of silica gel, and reduces the transition adsorption and dead adsorption of highly polar and alkaline analytes.
ClaricepTMPerformance comparison between Flash CM deactivated silicone and ordinary deactivated silicone
Flash Column: ClaricepTMFlash CS 12 g
Sample: catechol 100 mg/mL
Mobile phase: dichloromethane: methanol=98:2
Detection wavelength: 254 nm
Injection volume: 20 μ L
ClaricepTMFlash amino column
ClaricepTMFlash amino columns are supported on silica gel and bonded with propylamine functional groups. These fillers belong to polar stationary phases and weak anion exchangers, with dual functions and unique retention properties. In organic solvent systems, it can be combined with - OH- NH， Or - SH functional groups form hydrogen bonds in the analyte. It can also be used as a weak anion exchanger under acidic conditions to remove sulfonic acid ions and strong anion interferences from the sample.
ClaricepTMFlash HILIC Column
Bona Aijer's amide column is compatible with both normal and reverse phase mobile phase systems, such as methanol, acetonitrile, and water, making it more convenient for solvent treatment than ordinary silica gel columns. At the same time, HILIC material and C18 material have opposite retention characteristics in the reverse phase system, with weaker retention than NH2 in the normal phase system, allowing for the elution or retention of substances that are difficult to elute under normal phase conditions or weakly retained under reverse phase conditions. ClaricepTMThe Flash HILIC column has good reproducibility, especially in the aqueous phase with excellent lifespan, which compensates for the traditional NH2 The shortcomings of the pillar.
Suitable for samples that are insoluble in non-polar solvents such as n-hexane, isopropanol, toluene, and dichloromethane
Suitable for retaining strong polar substances in silicone columns.
Suitable for mixed samples of various compounds with wide polarity (simultaneously separating non-polar, weakly polar, and strongly polar analytes)
Specific surface area: 300 m2/g. Aperture: 100 Å; Moisture content: 3% -5%; Average particle size: 40-60 μ m;
Chromatographic column: ClaricepTMFlash HILIC，20 g
Sample: 10 mg of uracil dissolved in 3 mL of hot water
Mobile phase: water;
Wavelength: 254 nm;
Flow rate: 12 mL/min
Sample: Direct solid loading of 100 mg VC and 70 mg VB2
Mobile phase: 0-2 min: ethyl acetate;
2-22 min: Methanol (0-100%);
22-52 min: Methanol
Wavelength: 280 nm
Flow rate: 25 mL/min
Sample: 100mg VC and 70mg VB2 were directly loaded onto solid samples
Mobile phase: 1% tetrahydrofuran: acetonitrile=99:1
Wavelength: 280 nm
Flow rate: 25 mL/min
ClaricepTMFlash Reverse Column
Reverse phase packing bonds non-polar alkyl functional groups such as C4, C8, C18, etc. This reverse phase separation mode is opposite to the normal phase. In reverse phase chromatography, non-polar or hydrophobic compounds are strongly retained, while polar samples are weakly retained, allowing for faster passage through the column bed. Due to its good reproducibility and wide range of applications, the use of reverse phase packing is becoming a popular purification and separation technology.
ClaricepTMFlash C18 Column
C18 columns can supply two types of finished columns with different pore sizes, with the following parameters:
Specific surface area: 300 m2/g. Pore size: 100 Å, average particle size: 40-60 μ m, carbon content: 14%;
Chromatographic column: ClaricepTMFlash C18，60 Å，40~60 μm，330 g；
Mobile phase: A： 0.01% formic acid aqueous solution; B： 0.01% formic acid acetonitrile solution;
Wavelength: UV 254 nm、280 nm； Flow rate: 100 mL/min;
Injection volume: 10 mL
